富血小板血浆(PRP)作为一种再生剂已被广泛应用于各种组织的血管化。

              尽管如此，大部分与PRP中发现的抗凝血剂的额外使用有关的缺点已经被证明抑制了伤口的愈合过程。

             由于这些原因，利用较低的离心速度，研制了一种新型的无添加剂的血小板浓缩液。

             因此，本研究的目的是研究这种新疗法的成骨细胞行为(注射性富含血小板的纤维蛋白；I-PRF，100%天然，不含添加剂)与传统的PRP相比。用I-PRF或PRP培养人原代成骨细胞，并与对照组织培养塑料进行比较。

            采用活/死法、迁移法和细胞粘附/增殖法。此外，用碱性磷酸酶(ALP)、茜素红和骨钙素染色，以及实时PCR技术检测Runx 2、ALP、胶原1和骨钙素基因对成骨细胞的分化作用。

            结果表明，无论培养条件如何，所有细胞在整个研究期间都有较高的存活率。PRP诱导成骨细胞迁移显著增加2倍，而I-PRF则比对照组织培养塑料和PRP增加3倍。与PRP相比，I-PRF在第3天和第5天诱导细胞增殖率明显高于PRP。

            此外，I-PRF在第7天诱导ALP染色显著增加，在14d诱导茜素红染色增强。与PRP相比，I-PRF组ALP、Runx 2和骨钙素mRNA水平及骨钙素免疫荧光染色均显著升高。

            总之，与传统的抗凝血剂相比，本研究的结果更有利于天然配方的i-prf的使用。因此，有必要进一步研究I-PRF中纤维蛋白和白细胞的直接作用，以便更好地阐明它们在组织创伤愈合中的积极作用。

Effects of an injectable platelet-rich fibrin on osteoblast behavior and bone tissue formation in comparison to platelet-rich plasma - PubMed

             Platelet-rich plasma (PRP) has been utilized for many years as a regenerative agent capable of inducing vascularization of various tissues using blood-derived growth factors.

             Despite this, drawbacks mostly related to the additional use of anti-coagulants found in PRP have been shown to inhibit the wound healing process.

             For these reasons, a novel platelet concentrate has recently been developed with no additives by utilizing lower centrifugation speeds.

             The purpose of this study was therefore to investigate osteoblast behavior of this novel therapy (injectable-platelet-rich fibrin; i-PRF, 100% natural with no additives) when compared to   traditional PRP. Human primary osteoblasts were cultured with either i-PRF or PRP and compared to control tissue culture plastic.

            A live/dead assay, migration assay as well as a cell adhesion/proliferation assay were investigated. Furthermore, osteoblast differentiation was assessed by alkaline phosphatase (ALP), alizarin red and osteocalcin staining, as well as real-time PCR for genes encoding Runx2, ALP, collagen1 and osteocalcin.

            The results showed that all cells had high survival rates throughout the entire study period irrespective of culture-conditions. While PRP induced a significant 2-fold increase in osteoblast migration, i-PRF demonstrated a 3-fold increase in migration when compared to control tissue-culture plastic and PRP.

             While no differences were observed for cell attachment, i-PRF induced a significantly higher proliferation rate at three and five days when compared to PRP.

              Furthermore, i-PRF induced significantly greater ALP staining at 7 days and alizarin red staining at 14 days. A significant increase in mRNA levels of ALP, Runx2 and osteocalcin, as well as immunofluorescent staining of osteocalcin was also observed in the i-PRF group when compared to PRP.

              In conclusion, the results from the present study favored the use of the naturally-formulated i-PRF when compared to traditional PRP with anti-coagulants.

              Further investigation into the direct role of fibrin and leukocytes contained within i-PRF are therefore warranted to better elucidate their positive role in i-PRF on tissue wound healing.