来自PubMed文献索引
摘要
目标：本研究的目的是评价注射型富含纤维蛋白血小板(I-PRF)的血细胞含量、形态学、Ⅰ
型胶原基因表达和生长因子的释放。
材料和方法：从15名志愿者中采集血液样本，制备I-PRF样本.以外周血为对照组。血凝块
和I-PRF标本培养10天.取培养上清液，ELISA免疫法测定PDGF和VEGF生长因子在1、8、
24、72和240 h的表达，用组织学和免疫组织化学方法检测IL-10、骨钙素和转化生长因子-
β的生物学特性。用扫描电镜(SEM)观察血小板和白细胞的纤维蛋白网络和分布。采用逆转
录聚合酶链反应(RT-PCR)方法检测Ⅰ型胶原基因的表达.
结果：I-PRF组血小板和淋巴细胞浓度高于外周血(P<0.05)。血凝块中VEGF的释放量
(1933±704)高于I-PRF(852±376，p<0.001)。免疫组化显示I-PRF组TGF-B、IL-10和骨钙
素表达上调.RT-PCR显示I型胶原基因在I-PRF中表达增加(p<0.05).扫描电镜图像显示血小
板在某些区域聚集，而纤维蛋白网络在整个I-PRF样品中明显可见。
结论：注射性血小板丰富纤维蛋白是一种良好的软组织愈合和矿化的方法，考虑到三维纤
维蛋白网络的形成，包括血小板、白细胞、I型胶原、骨钙素和生长因子。事实上，可注射
的富含纤维蛋白的血小板可以在生物活性、简化技术以及与其他生物材料的流动混合等医
学应用中得到证实。
临床相关性：富含血小板纤维蛋白的形态、细胞和蛋白质特性为临床应用提供了更好的认
识和完善的临床指导方针。一旦具有良好的物理化学和生物学特性，可注射的富含血小板
纤维蛋白的应用可扩展到骨科、牙周学和种植牙科等领域的软组织和矿化组织修复过程
中。
Injectable platelet rich fibrin: cell content, morphological, and protein characterization - PubMed
Abstract
Objectives: The aim of the present study was to evaluate the blood cell content,
morphological aspects, gene expression of type I collagen, and release of growth
factors on an injectable platelet rich fibrin (i-PRF).
Materials and methods: Blood samples were collected from 15 volunteers to
prepare i-PRF samples. Peripheral blood was used as a control group. Blood clot
and i-PRF samples were cultured for 10 days. The supernatant of the samples was
collected for ELISA immunoassay quantification of PDGF and VEGF growth factors
over periods of 1, 8, 24, 72, and 240 h. I-PRF and blood clot samples were
biologically characterized using histological and immunohistochemistry analysis
for
IL-10, osteocalcin, and TGF-β. Scanning electron microscopy (SEM) was used to
inspect the fibrin network and distribution of blood platelets and leukocytes.
Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to
evaluate gene expression for type I collagen.
Results: A higher concentration of platelets and lymphocytes was recorded in i-
PRF than in peripheral blood (p < 0.05). The release of VEGF was higher in blood
clot samples (1933 ± 704) than that for i-PRF (852 ± 376; p < 0.001).
Immunohistochemistry showed upregulation of TGF-B, IL-10, and osteocalcin in
the i-PRF group. RT-PCR showed increased type I collagen gene expression in i-
PRF (p < 0.05). SEM images revealed agglomeration of platelets in some regions,
while a fibrin networking was noticeable in the entire i-PRF sample.
Conclusions: Injectable platelet rich fibrin becomes a good approach for soft and
mineralized tissue healing considering the formation of a three-dimensional fibrin
network embedding platelets, leukocytes, type I collagen, osteocalcin, and growth
factors. Indeed, the injectable platelet rich fibrin can be indicated in several
medical
applications regarding bioactivity, simplied technique, and flowable mixing with
other biomaterials.
Clinical relevance: Morphological, cell, and protein characterization of platelet
rich
fibrin provides a better understanding of the clinical effects and improvement of
clinical guidelines for several medical applications. Once well physicochemical and
biologically characterized, the use of an injectable platelet rich fibrin can be
extended to other applications in the field of orthopedics, periodontics, and
implant dentistry on the repairing process of both soft and mineralized tissues.
